mmp14 protein expression  (Human Protein Atlas)

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Single-cell transcriptome analysis of the relationship between MMP14 and colorectal cancer. (A) CD45 negative non-immune cell annotated tSNE; (B) Expression of MMP14 in different cell subsets of tSNE; (C) The expression of MMP14 among different cell subsets; (D) Fibroblasts subgroup subdivision; (E) The expression of MMP14 in different subpopulations after Fibroblasts subgroups; (F) Fib6 ratio among different samples; (G) Fib6 and other Fib difference analysis volcano map; (H) GO enrichment analysis of differential genes; (I) KEGG enrichment analysis of differential genes.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Single-cell transcriptome analysis of the relationship between MMP14 and colorectal cancer. (A) CD45-positive immune cell annotated tSNE; (B) Histogram of the proportional accumulation of immune cells between different samples; (C) A 50-point bitmap of the significant difference of immune cells between the two groups. Statistical significance is denoted as **P < 0.01. ns means no significant difference, p greater than 0.05.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques:

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Development of prognostic gene markers based on MMP14-associated co-expression genes and MMP14. (A) Univariate Cox regression analysis to assess prognostic significance; (B, C) LASSO assays for feature selection; (D) Multivariate Cox regression analysis to construct prognostic model; (E, F) Evaluation of the impact of the prognostic model on OS and PFS; (G) ROC curves for model validation. (H-J) Heat map illustrating expression patterns of six genes, the distribution of risk score, and the survival status of CRC patients.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Expressing, Selection, Construct, Biomarker Discovery

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Experimental detection of MMP14 expression in clinical samples. (A) qRT-PCR for MMP14 expression detection; (B) Western blot for detection of MMP14 expression; (C) Immunofluorescence for the detection of MMP14 expression, with DAPI staining for cellular localization; (D) H&E staining of three pairs of cancerous and adjacent tissues. Statistical significance is denoted as *P < 0.05, **P < 0.01.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

Journal: Frontiers in Oncology

Article Title: Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer

doi: 10.3389/fonc.2025.1564375

Figure Lengend Snippet: Construction of MMP14-silenced CRC cell system. (A) qRT-PCR detection of IL-1 β mRNA expression; (B) Knockdown of MMP14 detected by qRT-PCR; (C) Flow cytometry was used to detect cell apoptosis following MMP14 silencing. Statistical significance is denoted as **P < 0.01, and ****P < 0.0001.

Article Snippet: Additionally, to further examine MMP14 protein expression levels in CRC, we examined the Human Protein Atlas (HPA) database, focusing on MMP14 protein expression in various malignancies. ( www.proteinatlas.org/ ).

Techniques: Quantitative RT-PCR, Expressing, Knockdown, Flow Cytometry

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: Gene expression levels of MMP14 in TCGA combined GTEx database. (A) Quantitative difference of MMP14 expression based on pan-cancer analysis. (B) Quantitative difference of MMP14 expression in the TCGA-KIRC dataset combined with the GTEx-kidney dataset. (C) The ROC curve of MMP14 expression between tumor and normal tissue in TCGA-KIRC combined GTEx-kidney dataset. (D) Quantitative difference of MMP14 expression in TCGA-KIRC paired samples. ns, P≥0.05; *, P<0.05; **, P<0.01; ***, P<0.001. Red represents upregulation, and blue represents downregulation. MMP14, matrix metalloproteinase 14; TPM, transcripts per million; TCGA, The Cancer Genome Atlas; GTEx, Genotype-Tissue Expression; ns, not statistically; KIRC, kidney renal clear cell carcinoma; ROC, receiver operating characteristic; AUC, area under the curve; CI, confidence interval; TPR, true positive rate; FPR, false positive rate.

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques: Gene Expression, Expressing

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: Relationship between MMP14 expression level and clinicopathological variables in KIRC patients

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques: Expressing

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: Univariate and stepwise multivariate Cox hazard analysis of MMP14 in KIRC

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques:

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: Prognostic analysis of MMP14 in TCGA database. (A-C) Univariate Cox regression prognostic analysis based on TCGA pancancer analysis. The X-axis represents HR and 95% CI, Y-axis represents cancer type, the circle size represents −log 10 P value, and the color of the circle represents risk type. (D-F) Kaplan-Meier survival analysis of MMP14 in the TCGA-KIRC dataset. (G-I) Time-dependent ROC analysis of MMP14 in the TCGA-KIRC dataset. OS, overall survival; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; NS, not statistically; DSS, disease-specific survival; PFI, progression-free interval; HR, hazard ratio; CI, confidence interval; MMP14, matrix metalloproteinase 14; ROC, receiver operating characteristic; AUC, area under the curve; FPR, false positive rate.

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques:

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: Disease ontology GSEA. (A) Kidney cancer-related diseases from the DO database. (B) Kidney cancer-related diseases from the DisGeNET database. DO, Disease Ontology; GSEA, gene set enrichment analyses; MMP14, matrix metalloproteinase 14; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; NES, neuroendocrine syndrome.

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques:

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: KEGG signaling pathway GSEA. (A) The dotplot of top 12 KEGG gene set analyses results. (B) The gene-concept network for top 5 KEGG gene set analyses results. (C) The concept-concept network for top 30 KEGG gene set analyses results. KEGG, Kyoto Encyclopedia of Genes and Genomes; GSEA, gene set enrichment analyses; MMP14, matrix metalloproteinase 14; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; COVID-19, coronavirus disease 2019.

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques:

Journal: Translational Andrology and Urology

Article Title: Upregulation of matrix metalloproteinase 14 (MMP14) is associated with poor prognosis in renal clear cell carcinoma—a bioinformatics analysis

doi: 10.21037/tau-22-619

Figure Lengend Snippet: Protein-protein interaction network of genes significantly and positively associated with MMP14 in KIRC. MMP14, matrix metalloproteinase 14; KIRC, kidney renal clear cell carcinoma.

Article Snippet: In the present study, we first investigated the messenger RNA (mRNA) and protein expression of MMP14 in KIRC from The Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) database and the association between MMP14 expression and survival prognosis in KIRC.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: B16F1 cell proliferation and migration on MMP14 Sf−/− and MMP14 Sf+/+ fibroblast matrix. ( a ) Schematic representation of fibroblast culture to obtain their matrix (picture of Coomassie-stained matrix after fibroblast removal) and seeding B16F1 cells on it. ( b ) BrdU incorporation measurement of B16F1 cells cultured on fibroblast matrix. ( c ) Colony outgrowth assay showing melanoma migration on fibroblast matrix (left) and quantification (right). Experiments were repeated three times. mWT (MMP14 Sf+/+ matrix, control matrix) n = 3; mSF −/− (MMP14 Sf−/− matrix) n = 3; continuous line, cell front after 0 h; dashed line, cell front after indicated period of time; * p < 0.05; *** p < 0.001; scale, 100 µm.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: Migration, Staining, BrdU Incorporation Assay, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: Analysis of MMP14 Sf−/− and MMP14 Sf+/+ fibroblast matrices. ( a ) Mass Spectrometric and ( b ) immunoblot analysis of fibroblast matrix and conditioned medium (8% SDS-PAGE). ( c ) Transcriptional analysis of fibroblast lysates for collagens VI (COL6A1) and XIV (COL14A1). ( d ) In vitro processing of recombinant collagen XIV (Coll XIV) and human MMP14 visualized on immunoblot and silver staining (4–12% Bis-Tris gradient gel). Collagen XIV fragments generated by MMP14 are indicated with black arrows. MMP14 Sf+/+ (WT), n = 3; MMP14 Sf−/− (SF −/− ), n = 3; pro-MMP14, inactive form of MMP14; * p < 0.05.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: Western Blot, SDS Page, In Vitro, Recombinant, Silver Staining, Generated

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: Melanoma cells proliferation on collagen XIV coatings. BrdU incorporation analysis of B16F1 cells cultured on ( a ) collagen XIV (Coll XIV) (1 to 10 µg/mL) and ( b ) on serum (FCS) alone and in combination with collagen XIV (1 to 10 µg/mL). ( c ) Proliferation was measured in cells cultured on matrix from control fibroblasts (mWT) alone or with the addition of collagen XIV (1 to 10 µg/mL) and MMP14 Sf−/− (SF −/− ) fibroblasts. Experiments were repeated three times. mWT (MMP14 Sf+/+ matrix, control matrix), n = 3; mSF −/− (MMP14 Sf−/− matrix), n = 3; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: BrdU Incorporation Assay, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: B16F1 cell migration on MMP14 Sf+/+ fibroblast matrix enriched with collagen XIV. Colony outgrowth assay on MMP14 Sf+/+ fibroblast matrix (mWT) containing 10 µg/mL collagen XIV (Coll XIV). The FCS-coated surface was used as a control. Below is the quantification in the percentage of the migrated area. Experiments were repeated three times. mWT (MMP14 Sf+/+ matrix), n = 3; FCS, n = 3; a continuous line marks the cell border at time 0 h (this time point is shown in the insert); dashed line, cells border after indicated period of time; * p < 0.05; scale: 100 µm.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: Migration

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: Analysis of B16F1 cell adhesion on collagen XIV coating and fibroblast matrix. ( a ) Melanoma adhesion assay on collagen XIV (Coll XIV) coatings (1 µg/mL; 10 µg/mL), ( b ) wildtype fibroblast matrix (mWT), in combination with 10 µg/mL collagen XIV (mWT + Coll XIV) and MMP14 Sf−/− matrix (mSF −/− ). Fibronectin (FN) was used as positive control. Experiments were repeated three times. mWT (MMP14 Sf+/+ matrix, control matrix), n = 3, mWT + Coll XIV, n = 3; mSF −/− (MMP14 Sf−/− matrix), n = 3; * p < 0.05; **** p < 0.0001; scale, 100 µm.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: Cell Adhesion Assay, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: Collagen XIV expression in mouse melanoma. Immunofluorescence staining of collagen XIV (red) and DAPI (blue) in melanoma from MMP14 Sf+/+ and MMP14 Sf−/− mice. The graph represents quantified signal intensity per area measured within peritumoral tissue (100 µm radius from the tumor). MMP14 Sf+/+ (WT), n = 6; MMP14 Sf−/− (SF −/− ), n = 5; t, tumor; e, epidermis. ** p < 0.01; scale, 100 µm.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: Expressing, Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Matrix Remodeling by Fibroblast-MMP14 Regulates Melanoma Growth

doi: 10.3390/ijms222212276

Figure Lengend Snippet: Analysis of collagen XIV and MMP14 expression in human tissue samples. Immunofluorescence detection of collagen XIV (red) and immunohistochemical staining for MMP14 in human nevi and melanoma. The nuclei are in blue (DAPI). Average collagen XIV signal intensity per area is shown on the left. Yellow arrowheads, nests of melanocytic nevi; white/black arrowheads, cutaneous melanoma nests; e, epidermis; n, nevus; t, tumor; s, stroma; scale, 100 µm.

Article Snippet: Recombinant human MMP14 100 ng/µL (918-MPN, R&D Systems, Wiesbaden, Germany) was activated using 0.1 µg/mL trypsin (15090046, Thermo Fisher Scientific, Darmstadt, Germany) in 50 mM Tris, 0.15 M NaCl, 10 mM CaCl 2 , 5 µM ZnCl 2 , 0.05% Brij-35, pH 7.5 for 1 h at 37 °C.

Techniques: Expressing, Immunofluorescence, Immunohistochemical staining, Staining